: Ensure both Windows Sound Settings and your DAW are set to the same sample rate (e.g., 48000Hz). Software & Driver Solutions Error Code 0x54f - Cubase - Steinberg Forums

To fix the problem effectively, it helps to know why it's happening. Based on user reports and Focusrite support documentation, there are several common triggers:

The "Cannot open Focusrite USB ASIO. (Error code: 0x54f)" error is undoubtedly a frustrating roadblock, but it is rarely an insurmountable one. It almost always signals a solvable issue, typically stemming from driver conflicts, outdated software, simple connection problems, or an overlooked sample rate mismatch. By methodically following the steps outlined in this guide—from updating and reinstalling your official Focusrite drivers and removing conflicting drivers like ASIO4ALL, to verifying your physical USB connection, optimizing your Windows power settings, and ensuring sample rate consistency—you have the tools to diagnose and resolve the problem. We hope this article has provided you with a clear path back to a stable, low-latency audio environment, allowing you to return to your creative work without further disruption.

A very common solution to the 0x54f error is to uninstall any generic or third-party ASIO drivers that may be conflicting with your Focusrite's native driver.