Plant Tissue Culture Ppt Pdf Extra Quality -

, Gamborg (B5), and White's medium. These contain essential macronutrients, micronutrients, vitamins, and a carbon source (usually sucrose). Growth Regulators : The balance between (promoting root growth) and cytokinins

A Latin term meaning "in glass," referring to experiments raised outside a living organism in test tubes, flasks, or petri dishes. plant tissue culture ppt pdf

The apical or axillary meristematic dome is excised and cultured. , Gamborg (B5), and White's medium

The goal here is to increase the number of shoots. The tissue is repeatedly subcultured (divided and transferred to fresh medium) containing specific plant hormones to stimulate lateral budding and multiple shoot formation. Stage 3: Rooting (In Vitro or Ex Vitro) The apical or axillary meristematic dome is excised

The balance between Auxins (roots) and Cytokinins (shoots) dictates the plant’s development.

A small piece of tissue (explant)—such as a leaf, stem segment, root, or bud—is excised from the mother plant.

Gradually acclimatizing the plantlets to lower humidity and higher light intensities before field transfer. 4. Components of Tissue Culture Media